Title: PHYTOCHEMICAL ANALYSIS AND PHARMACOLOGICAL EVALUATION OF LEAF OF LANNEA COROMANDELICA LINN.
Author(s): Tekeshwar Kumar and Vishal Jain
WHO is highly recommended the customary exploitation of traditional therapeutics of medicinal plants and is consequently in pipeline for development of monograph and exploring their uncultivated pharmacological properties. Presently, correct identification of medicinal plants by phytochemical fingerprints is a speedy tool to ensure reproducible quality of herbal drugs. In our study, the ethyl acetate and methanol extract of leaves of Lannea coromandelica (L. coromandelica) was subjected for chemical fingerprint as well therapeutic aid as antifilarial agents. High performance thin layer chromatography (HPTLC) was employed to develop chemical fingerprint and filariciadal activity was assessed by motility inhibition and MTT reduction assay with concentrations range 1000 to 25µg/ml. The HPTLC analysis of ethyl acetate and methanol extract was carried out using hexane: ethyl acetate: formic acid: methanol (5:4:1:0.5 v/v/v/v) for fingerprinting and quantification ellagic acid and quercetin. The HPTLC method was found to give compact spots for Rf = 0.54 and 0.51 ellagic acid; Rf = 0.91 and 0.87 quercetin for ethyl acetate and methanol extract respectively. The HPTLC method was validated as per the ICH guidelines. Inhibitory concentration (IC50) for the ethyl acetate extract was found to be 284.5µg/ml. In motility assay, complete inhibition of motility was observed for all concentrations. Hence, our study could be valuable for inventing strategies for quality control parameter and justifies the ethnic uses of plant in folkloric medicines.
Keywords: WHO, Lannea coromandelica, HPTLC, filariciadal activity.