Title: DESIGN AND IN VITRO CHARACTERIZATION OF STAVUDINE LOADED NON IONIC SURFACTANT VESICLES
Author(s): Prakash Goudanavar, Gobinda Kumar, Doddayya Hiremath and Jayadev Hiremath
The present investigation was aimed to prepare, characterize and optimize stavudine loaded nonionic surfactant vesicles (niosomes) for improvement in therapeutic index and efficacy of stavudine. Stavudine loaded niosomes were prepared by ether injection method, thin film hydration method, sonication method and reverse phase evaporation method using cholesterol and different surfactants. The formulations were then characterized with respect to vesicle size, entrapment efficiency, in vitro drug release profile and stability under specific conditions of temperature and humidity.Vesicles formed were descrete and spherical. Vesicles formed with tweens were larger in size compared to vesicles of spans. The entrapment efficiency of the formulations was found in between 60.12%-80.56%.Higher entrapment efficiency was observed with the formulations prepared from spans. The formulation T2 showed the highest entrapment efficiency with 80.54%. The cumulative percent drug release was in the range of 59.70% to 81.21% in 24 hrs. Formulation T3 shown highest cumulative percent drug release of 81.21%. Stability studies indicated 4-8ºC was the most suitable condition for storage of Stavudine loaded niosomes.The results of the study revealed that stavudine loaded niosomes are capable of releasing the drug for extended periods of time.
Keywords: Stavudine, Niosomes, thin film hydration method, sonication in vitro, stability study.