CYTOTOXIC AND GROWTH INHIBITORY EFFECTS OF CAMELLIA SINENSIS LINN. (THEACEAE) LEAF METHANOL EXTRACT ON TADPOLES AND GUINEA CORN RADICLE BY BENCH-TOP BIO-ASSAY

Title: CYTOTOXIC AND GROWTH INHIBITORY EFFECTS OF CAMELLIA SINENSIS LINN. (THEACEAE) LEAF METHANOL EXTRACT ON TADPOLES AND GUINEA CORN RADICLE BY BENCH-TOP BIO-ASSAY

Author(s): UKWUBILE Cletus A

ABSTRACT

Camellia sinensis is indicated in traditional herbal medicine as one of the plant used in treatment of tumor-related ailments in China and India. Cytotoxic and growth inhibitory effects of the methanol extract of leaf was evaluated using the tadpoles of Raniceps raninnus and the radicle length of Sorghum bicolor seeds respectively. The methanol extract of the leaf produced 70% mortality at a concentration of 200mg/mL and was eventually increased to 90% mortality at the concentration of 400mg/mL over a period of 2 h in the tadpoles. An average growth length of 7.0±2.4mm was produced by the radicles in the control seeds after 24 h with reduction in values   to 5.0±2.0, 6.1±2.0, 4.2 ±0.5, 3.1±0.5, 2.3±0.1 and 0.1±0.01 in the seeds treated with 2.0, 2.5, 3.0, 3.5, 4.0, 4.5 and 5.0 mg/mL of the extract concentrations. At 96h, the control seeds recorded a total length of 35.1±12.1 mm in relation to 13.0±5.5, 11.1±4.3, 8.5±3.0, 4.9±10, 4.3±0.1, 3.7±0.4 and 2.4±1.3 produced by the seeds treated with 2.0, 2.5, 3.0, 3.5, 4.0, 4.5 and 5.0 mg/mL of the leaf extract respectively. The results justified the ethnomedicinal claimed of the plant in the treatment of tumors and cancer.

KEYWORDS: Camellia sinensis, cytotoxic, Raniceps raninnus, Sorghum bicolor.

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