Title: AN IN VIVO IMAGING OF CUTANEOUS LEISHMANIASIS IN BALB/C MICE
Author(s): Basma Doro, Najib M Sufya, Fadia Mohamed Gafri and K. C. Carter
Human leishmaniasis is a spectrum of diseases caused by protozoan parasites of the genus Leishmania. The disease affects the populations of 88 counties of the tropics and sub-tropics with pathologies ranging from cutaneous and muco-cutaneous lesions to visceral disease. L. major infection causes cutaneous disease and is characterised by skin lesions. Previous studies have shown that it is possible to monitor leishmanial infection in the same mice by monitoring the bioluminescent signal from mice given parasites expressing the luciferase gene. Therefore in this study the measurement of parasites burdens using bioluminescence correlated well with monitoring lesion size in infected L. major and L. mexicana infected mice and parasites grew better in the footpads compared to the rump. Infection with L. major caused a more aggressive infection compared to L. mexicana. In this study, there was a significant difference between rump and footpad infected withL. mexicana promastigotes in phagocytic cells present in the infected site as detected using the chemiluminescence emitted. However there was no significant between rump and footpad for mice infected with L. major promastigotes.In vivo imaging model of mice infected with luciferase expressing parasites is easy and convenient way to follow disease and parasite growth could be detected much earlier.
KEYWORDS: Bioluminescence imaging, leishmaniasis.